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SHR Neuro Cancer Cardio Lipid Metab Microb

DiNicola, M; Seuylemezian, A; Guan, L; Moissl-Eichinger, C; Baker, A; Johns, J.
Modeling of recovery efficiency of sampling devices used in planetary protection bioburden estimation.
Appl Environ Microbiol. 2023; e0083223 Doi: 10.1128/aem.00832-23 [OPEN ACCESS]
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Co-authors Med Uni Graz

Moissl-Eichinger Christine

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Abstract:

Planetary protection at the National Aeronautics and Space Administration (NASA) requires bioburden on certain spacecraft to be estimated via sampling in order to comply with biological cleanliness requirements. To achieve this, the recovery efficiency of devices used to sample the spacecraft pre-launch must be understood and their uncertainty quantified in order to produce the most reasonable estimates of bioburden. This study brings together experiments performed by NASA and the European Space Agency with approved swab and wipe sampling devices, inoculating steel coupons with laboratory strains of Bacillus spp. spores commonly recovered from spacecraft assembly clean rooms (B. atrophaeus, B. megaterium, B. safensis and B. thuringiensis), with a mathematical model of the assay process to assess recovery efficiency. The statistical treatment developed in this study allows comparison of bioburden estimates made from different devices processed by different methods. This study also gives stakeholders and practitioners a statistically rigorous approach to predict bioburden that can be folded into future modeling efforts.

Find related publications in this database (Keywords)

planetary protection

recovery efficiency

assay

bioburden

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