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SHR Neuro Cancer Cardio Lipid Metab Microb

Eigenfeld, M; Wittmann, L; Kerpes, R; Schwaminger, SP; Becker, T.
Studying the impact of cell age on the yeast growth behaviour of Saccharomyces pastorianus var. carlsbergensis by magnetic separation.
Biotechnol J. 2023; 18(7):e2200610 Doi: 10.1002/biot.202200610
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Leading authors Med Uni Graz

Eigenfeld Marco

Schwaminger Sebastian

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Abstract:

Despite the fact that yeast is a widely used microorganism in the food, beverage, and pharmaceutical industries, the impact of viability and age distribution on cultivation performance has yet to be fully understood. For a detailed analysis of fermentation performance and physiological state, we introduced a method of magnetic batch separation to isolate daughter and mother cells from a heterogeneous culture. By binding functionalised iron oxide nanoparticles, it is possible to separate the chitin-enriched bud scars by way of a linker protein. This reveals that low viability cultures with a high daughter cell content perform similarly to a high viability culture with a low daughter cell content. Magnetic separation results in the daughter cell fraction (>95%) showing a 21% higher growth rate in aerobic conditions than mother cells and a 52% higher rate under anaerobic conditions. These findings emphasise the importance of viability and age during cultivation and are the first step towards improving the efficiency of yeast-based processes.

Find related publications in this database (using NLM MeSH Indexing)

Saccharomyces cerevisiae - metabolism

Saccharomyces - administration & dosage

Cell Cycle - administration & dosage

Fermentation - administration & dosage

Magnetic Phenomena - administration & dosage

Find related publications in this database (Keywords)

bioseparation

fermentation

magnetic separation

physiological state

yeast

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