Selected Publication:

SHR Neuro Cancer Cardio Lipid Metab Microb

Osto, E; Castellani, C; Fadini, GP; Baesso, I; Gambino, A; Agostini, C; Avogaro, A; Gerosa, G; Thiene, G; Iliceto, S; Angelini, A; Tona, F.
Impaired endothelial progenitor cell recruitment may contribute to heart transplant microvasculopathy.
J Heart Lung Transplant. 2011; 30(1):70-6 Doi: 10.1016/j.healun.2010.07.004
Web of Science PubMed FullText FullText_MUG

 

Leading authors Med Uni Graz

Osto Elena

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Abstract:

BACKGROUND: Circulating progenitor cells (PCs) may play a role in the pathogenesis of cardiac allograft vasculopathy, the leading cause of morbidity and mortality in heart transplantation (HTx). We assessed the relationship between circulating PCs and their incorporation into allografts and coronary microvascular function in HTx. METHODS: PCs were quantified by flow cytometry on the basis of the surface expression of CD34, CD133, and kinase domain receptor (KDR) antigens. Biopsy specimens at 2 different times were examined. Immunohistochemistry for the stem cell marker c-Kit, endothelial PC (EPC) marker KDR, and CD34 was performed in serial sections in all specimens. Cells positive for each marker were counted in all specimen area sections, and the number obtained was corrected by area section. Coronary flow in the left anterior descending coronary artery was detected at rest and during intravenous adenosine by transthoracic echocardiography. Coronary flow reserve (CFR) was the ratio of hyperemic diastolic mean velocity (DMV)/resting DMV. RESULTS: CFR was measured in 29 patients and was abnormal (CFR < 2) in 6 (Group A) and normal in 23 (Group B). CFR was lower in Group A (1.5 ± 0.1 vs 3.3 ± 0.8, p < 0.0001). CD34(+)KDR(+), CD133(+)KDR(+), and CD34(+)CD133(+)KDR(+) cell counts were lower in Group A (p < 0.05). EPCs in biopsy sections tended to be lower in Group A (p = 0.06) and correlated to circulating CD133(+)KDR(+) and CD34(+)CD133(+)KDR(+) (p = 0.003 and p = 0.052, respectively). CONCLUSIONS: EPCs are decreased in the circulation and in the allograft in patients with microvasculopathy. Defective mobilization and engraftment of EPCs may be involved in the pathogenesis of cardiac allograft vasculopathy.

Find related publications in this database (using NLM MeSH Indexing)

AC133 Antigen - administration & dosage

Adult - administration & dosage

Antigens, CD - metabolism

Antigens, CD34 - metabolism

Blood Flow Velocity - administration & dosage

Cell Count - administration & dosage

Cell Movement - administration & dosage

Coronary Circulation - administration & dosage

Endothelium, Vascular - metabolism, pathology

Female - administration & dosage

Glycoproteins - metabolism

Heart Transplantation - adverse effects, pathology

Humans - administration & dosage

Male - administration & dosage

Microcirculation - administration & dosage

Middle Aged - administration & dosage

Peptides - metabolism

Stem Cells - metabolism, pathology

Transplantation, Homologous - administration & dosage

Vascular Diseases - etiology, physiopathology

Vascular Endothelial Growth Factor Receptor-2 - metabolism

Find related publications in this database (Keywords)

endothelial progenitor cell

heart transplantation

microvasculopathy

coronary flow reserve

cardiac allograft vasculopathy

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