Selected Publication:

SHR Neuro Cancer Cardio Lipid Metab Microb

Clarke, A; Groschner, K; Stockner, T.
Exploring TRPC3 Interaction with Cholesterol through Coarse-Grained Molecular Dynamics Simulations
BIOMOLECULES. 2022; 12(7): 890 Doi: 10.3390/biom12070890 [OPEN ACCESS]
Web of Science PubMed FullText FullText_MUG

 

Co-authors Med Uni Graz

Groschner Klaus

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Abstract:

Transient receptor potential canonical 3 (TRPC3) channel belongs to the superfamily of transient receptor potential (TRP) channels which mediate Ca2+ influx into the cell. These channels constitute essential elements of cellular signalling and have been implicated in a wide range of diseases. TRPC3 is primarily gated by lipids and its surface expression has been shown to be dependent on cholesterol, yet a comprehensive exploration of its interaction with this lipid has thus far not emerged. Here, through 80 mu s of coarse-grained molecular dynamics simulations, we show that cholesterol interacts with multiple elements of the transmembrane machinery of TRPC3. Through our approach, we identify an annular binding site for cholesterol on the pre-S1 helix and a non-annular site at the interface between the voltage-sensor-like domain and pore domains. Here, cholesterol interacts with exposed polar residues and possibly acts to stabilise the domain interface.

Find related publications in this database (Keywords)

cholesterol

TRPC3

molecular dynamics simulations

lipid-protein interactions

annular lipids

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