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SHR Neuro Cancer Cardio Lipid Metab Microb

Merz, J; Nettesheim, A; von, Garlen, S; Albrecht, P; Saller, BS; Engelmann, J; Hertle, L; Schäfer, I; Dimanski, D; König, S; Karnbrock, L; Bulatova, K; Peikert, A; Hoppe, N; Hilgendorf, I; von, Zur, Mühlen, C; Wolf, D; Groß, O; Bode, C; Zirlik, A; Stachon, P.
Pro- and anti-inflammatory macrophages express a sub-type specific purinergic receptor profile.
Purinergic Signal. 2021; 17(3):481-492 Doi: 10.1007/s11302-021-09798-3 [OPEN ACCESS]
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Co-authors Med Uni Graz

Peikert Alexander

Zirlik Andreas

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Abstract:

Extracellular nucleotides act as danger signals that orchestrate inflammation by purinergic receptor activation. The expression pattern of different purinergic receptors may correlate with a pro- or anti-inflammatory phenotype. Macrophages function as pro-inflammatory M1 macrophages (M1) or anti-inflammatory M2 macrophages (M2). The present study found that murine bone marrow-derived macrophages express a unique purinergic receptor profile during in vitro polarization. As assessed by real-time polymerase chain reaction (PCR), Gαs-coupled P1 receptors A2A and A2B are upregulated in M1 and M2 compared to M0, but A2A 15 times higher in M1. The ionotropic P2 receptor P2X₅ is selectively upregulated in M1- and M2-polarized macrophages. P2X₇ is temporarily expressed in M1 macrophages. Metabotropic P2Y receptors showed a distinct expression profile in M1 and M2-polarized macrophages: Gαq coupled P2Y₁ and P2Y₆ are exclusively upregulated in M2, whereas Gαi P2Y₁₃ and P2Y₁₄ are overexpressed in M1. This consequently leads to functional differences between M1 and M2 in response to adenosine di-phosphate stimulation (ADP): In contrast to M1, M2 showed increased cytoplasmatic calcium after ADP stimulation. In the present study we show that bone marrow-derived macrophages express a unique repertoire of purinergic receptors. We show for the first time that the repertoire of purinergic receptors is highly flexible and quickly adapts upon pro- and anti-inflammatory macrophage differentiation with functional consequences to nucleotide stimulation.

Find related publications in this database (using NLM MeSH Indexing)

Animals - administration & dosage

Cell Polarity - physiology

Cells, Cultured - administration & dosage

Inflammation Mediators - metabolism

Macrophages - metabolism

Mice - administration & dosage

Receptors, Purinergic - biosynthesis, genetics

Transcriptome - physiology

Find related publications in this database (Keywords)

Macrophages

Polarization

Purinergic receptor

Inflammation

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