Selected Publication:

SHR Neuro Cancer Cardio Lipid Metab Microb

Leis, HJ; Fauler, G; Windischhofer, W.
Enantioselective quantitative analysis of amphetamine in human plasma by liquid chromatography/high-resolution mass spectrometry.
Anal Bioanal Chem. 2014; 406(18):4473-4480 Doi: 10.1007/s00216-014-7850-4
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Leading authors Med Uni Graz

Leis Hans-Joerg

Co-authors Med Uni Graz

Fauler Günter

Windischhofer Werner

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Abstract:

A method for the quantitative enantioselective analysis of amphetamine in human plasma by LC-HRMS is presented. High-resolution detection, alone and in combination with targeted MS/MS, was validated and compared to a highly sensitive GC-NICI-Method. Derivatization with (S)-N-(heptafluorobutyryl)-prolyl chloride was accomplished to yield derivatives suitable for enantioselective analysis of amphetamine on a nonchiral reversed phase column with MS-compatible mobile phase. Equal analytical performance was observed for the methods presented and the GC-NICI-MS method. A dynamic range of 4,000 was found for the established calibration curves. A fivefold deuterated analogue of both enantiomeres was used as an internal standard. Full validation data are given to demonstrate the usefulness of the assay, including specificity, linearity, accuracy and precision, autosampler stability, matrix effect, and prospective analytical batch size accuracy. The method has been successfully applied to pharmacokinetic profiling of the drug after oral application.

Find related publications in this database (using NLM MeSH Indexing)

Administration, Oral -

Amphetamine - administration & dosage

Amphetamine - blood

Amphetamine - chemistry

Amphetamine - pharmacokinetics

Calibration -

Chromatography, Liquid - methods

Fluorocarbons - chemistry

Humans -

Proline - analogs & derivatives

Proline - chemistry

Reproducibility of Results -

Sensitivity and Specificity -

Stereoisomerism -

Tandem Mass Spectrometry - methods

Find related publications in this database (Keywords)

Bioanalytical methods

Chiral analysis

Drug monitoring

Drug screening

HPLC

Mass spectrometry

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