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SHR Neuro Cancer Cardio Lipid Metab Microb

Pabst, M; Fischl, RM; Brecker, L; Morelle, W; Fauland, A; Köfeler, H; Altmann, F; Léonard, R.
Rhamnogalacturonan II structure shows variation in the side chains monosaccharide composition and methylation status within and across different plant species.
Plant J. 2013; 76(1):61-72 Doi: 10.1111/tpj.12271 [OPEN ACCESS]
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Co-authors Med Uni Graz

Köfeler Harald

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Abstract:

A paradigm regarding rhamnogalacturonans II (RGII) is their strictly conserved structure within a given plant. We developed and employed a fast structural characterization method based on chromatography and mass spectrometry, allowing analysis of RGII side chains from microgram amounts of cell wall. We found that RGII structures are much more diverse than so far described. In chain A of wild-type plants, up to 45% of the l-fucose is substituted by l-galactose, a state that is seemingly uncorrelated with RGII dimerization capacity. This led us to completely reinvestigate RGII structures of the Arabidopsis thaliana fucose-deficient mutant mur1, which provided insights into RGII chain A biosynthesis, and suggested that chain A truncation, rather than l-fucose to l-galactose substitution, is responsible for the mur1 dwarf phenotype. Mass spectrometry data for chain A coupled with NMR analysis revealed a high degree of methyl esterification of its glucuronic acid, providing a plausible explanation for the puzzling RGII antibody recognition. The β-galacturonic acid of chain A exhibits up to two methyl etherifications in an organ-specific manner. Combined with variation in the length of side chain B, this gives rise to a family of RGII structures instead of the unique structure described up to now. These findings pave the way for studies on the physiological roles of modulation of RGII composition.

Find related publications in this database (using NLM MeSH Indexing)

Arabidopsis - chemistry

Cell Wall - metabolism

Chromatography, Liquid -

Fucose - analysis

Galactose - analysis

Hexuronic Acids -

Monosaccharides - chemistry

Mutation -

Organ Specificity -

Pectins - chemistry

Plant Leaves - chemistry

Spectrometry, Mass, Electrospray Ionization -

Find related publications in this database (Keywords)

rhamnogalacturonanII

structural analysis

Arabidopsis thaliana

plant cell wall

porous graphitic carbon

PGCC-ESI-MS

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