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SHR Neuro Cancer Cardio Lipid Metab Microb

Leis, HJ; Windischhofer, W; .
Quantitative determination of memantine in human plasma by GC using negative ion chemical ionization MS detection after derivatization with a new reagent, o-(pentafluorobenzyloxycarbonyl)-2,3,4,5-tetrafluorobenzoyl chloride.
MICROCHIM ACTA. 2012; 179(3-4): 249-255. Doi: 10.1007/s00604-012-0886-2
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Leading authors Med Uni Graz

Leis Hans-Joerg

Co-authors Med Uni Graz

Windischhofer Werner

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Abstract:

A method is presented for the quantitative determination of memantine in plasma by use of the derivatization reagent o-(pentafluorobenzyloxycarbonyl)-2,3,4,5-tetrafluorobenzoyl chloride. Memantine can be quantitatively analyzed down to 49 pg per mL of plasma using a 250 mu L sample and negative ion chemical ionisation mass spectrometry (GC-NICI-MS). Plasma samples were made alkaline with carbonate buffer and extracted with n-hexane. The extracts were treated with reagent solution for 20 min, concentrated, and submitted to GC-NICI-MS. The method is rapid because extraction and derivatization occur in one single step. Amantadine is used as an internal standard. The utility and robustness of the assay is demonstrated by giving data on specificity, linearity, accuracy and precision, benchtop stability, freeze-thaw stability, autosampler stability, aliquot analysis, and prospective analytical batch size accuracy.

Find related publications in this database (Keywords)

Memantine

NICI

GC-MS

PBTFBCl

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