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Improvement of MALDI-TOF identification of Pseudomonas species: A step towards fast and accurate investigation of antimicrobial susceptibility
- Abstract
- Pseudomonas that is widespread, especially in aquatic environments, and can survive well there under various conditions. Pseudomonas can cause infections in humans, animals and plants. The high natural resistance to antibiotics is a particular problem in human medicine. In recent years, the number of species from the genus has also increased considerably and studies suggest that many species remain to be described. To understand the importance of these different species for the environment but also for humans, a good and simple differentiation of the individual species is necessary.
The collaboration between ANSES and MUG will use MALDI TOF differentiation and an open database to develop a method for fast, cheap and reliable determination of species of Pseudomonas.
Strain collections of the two institutions serve as a basis. These include isolates from the environment, from human and veterinary samples. The isolates are to be chartered by sequencing and MALDI TOF. Resistance to important antibiotics will also be tested in these isolates using btoh methods Agar diffusion and Micro broth dilution. MALDI TOF specimens will be placed in a open accessible database. This application will be free of charge and can be used by anyone. Another advantage will be that with the application one can get in touch with other researchers working on Pseudomonas and thus a Pseudomonas network could be established
If this project proves successful, it could also be applied to other groups of bacteria (e.g. Aeromonas, Bacilli, etc.). In any case, this work lays the foundation for close collaboration between ANSES and MUG in the field of Pseudomonads research. The participating institutions in France and Austria have different and complementary expertise in the research of this genus. A harmonisation and an efficient exchange of methods will be a key factor for both, to deepen their standing in this field of research and to other aquatic genera like Vibrio, Aeromonas.
- Project Leader:
- Zarfel Gernot
- Duration:
- 01.01.2024-31.12.2025
- Programme:
- Wissenschaftlich-Technische Zusammenarbeit (WTZ)
- Type of Research
- basic research