Flow Cytometry Core Facility (closed)
Research fields, Methods and Equipment
Methods
- Activity of human thrombocytes and leukocytes (Multicolor analysis of activation markers)
- Apoptosis (DNA Sub-G1, Mitochondrial membrane potential, Annexin V vs.PI, Cleaved Caspase-3, TUNEL)
- Cell cycle (DNA staining, Phospho-Histone H3, Pyronin Y)
- Cell-based assays (Proliferation, cytotoxicity, viability, apoptosis)
- Cell/tissue culture (Establishment/culturing of primary cells and cell lines)
- Cellular proliferation (BrdU labeling)
- Clonal expansion (Single cell plate sorting (FACS))
- DNA ploidy (DNA content)
- for Core Facility services please check
www.medunigraz.at/zmf/facs
- Gene knockdown (RNA interference)
- Gene over-expression (Transient transfection (chemical, nucleofection))
- Immunophenotyping (Multicolor analysis of human blood cells)
- Mycoplasma detection (PCR, fluorescence microscopy)
- Protein analysis (cells and tissues) (Western blot, immunoprecipitation)
- Quantitation of multiple analytes (e.g., cytokines, phosphoproteins) (Luminex (xMAP))
- Quantitation of single analytes (e.g., cytokines) (ELISA)
- Regulatory T cells (Tregs) (Multicolor analysis of human and mouse Tregs)
- Single isolation of cells (Antigen expression (Magnetic bead sorting, MACS))
- Single/multiple sorting of cells (Antigen expression (FACS))
- Sorting of transfected cells (Fluorescent marker/antigen expression (FACS))
- Telomere length (Telomere Flow-FISH)
Equipment
autoMACS (Miltenyi Biotec)
Bio-Plex 200 (BioRad)
FACSAria (BD Biosciences)
FACSCalibur (BD Biosciences)
LSR II (BD Biosciences)
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