Abteilung Zentrum für Medizinische Forschung (ZMF)
Forschungsgebiete, Methoden, Ausstattung
Methoden
- Accurate Mass Determination (2ppm)
- Activity of human thrombocytes and leukocytes (Multicolor analysis of activation markers)
- Analysing the chemical composition of specimens at a high resolution, using analytical TEM. Main application is the research focus nanotoxicology, where it is necessary to follow the path of nanoparticles within tissues or cells.
- Analysing the fine structure of specimens using electron tomography, to detect changes in the shape and location of macromolecules within tissues or cells in 3D.
- Apoptosis (DNA Sub-G1, Mitochondrial membrane potential, Annexin V vs.PI, Cleaved Caspase-3, TUNEL)
- Cell cycle (DNA staining, Phospho-Histone H3, Pyronin Y)
- Cell-based assays (Proliferation, cytotoxicity, viability, apoptosis)
- Cell/tissue culture (Establishment/culturing of primary cells and cell lines)
- Cellular proliferation (BrdU labeling)
- Clonal expansion (Single cell plate sorting (FACS))
- DNA ploidy (DNA content)
- El Spectrum
- for Core Facility services please check
www.medunigraz.at/zmf/facs
- for Core Facility services please check
www.medunigraz.at/zmf/mb
- for Core Facility services please check
www.medunigraz.at/zmf/mi
- for Core Facility services please check
www.medunigraz.at/zmf/ms/proteomics.html
- for Core Facility services please check
www.medunigraz.at/zmf/ua
- Gene knockdown (RNA interference)
- Gene over-expression (Transient transfection (chemical, nucleofection))
- genome-wide DNA (arrayCGH, LOH/CNV, linkage studies)
- Histology and Staining/ Antigen retrieval
- Histology and Staining/ Automated embedding
- Histology and Staining/ Sectioning
- Histology and Staining/ Staining (immunohistochemical, routine stains)
- Histology and Staining/ Toxicity testing (viability, proliferation, apoptosis, micronucleus, thrombocyte activation, clotting, complement activation)
- Histology and Staining/ Visualization of organelles
- Immunophenotyping (Multicolor analysis of human blood cells)
- Isotopic Pattern Determination
- Lipid Profiling/ Ceramides
- Lipid Profiling/ Cholesterol
- Lipid Profiling/ Cholesterolester
- Lipid Profiling/ Diacylglycerols
- Lipid Profiling/ Dimethylphosphatidylethanolamine
- Lipid Profiling/ Fatty Acids
- Lipid Profiling/ Hydroxyceramides
- Lipid Profiling/ Lysophospholipids
- Lipid Profiling/ Phoasphatidylethanolamine
- Lipid Profiling/ Phosphatidylcholine
- Lipid Profiling/ Sphingomyeline
- Lipid Profiling/ Triacylglycerols
- Mass Determination
- Microscopy/ Co-localization studies
- Microscopy/ Conventional light and fluorescence microscopy
- Microscopy/ Intracellular ion changes (e.g. Ca2+)
- Microscopy/ Long -term live cell imaging (e.g.migration)
- MSn
- Mycoplasma detection (PCR, fluorescence microscopy)
- Post-Imaging Processing and Analysis/ Determination of intracellular ion concentrations
- Post-Imaging Processing and Analysis/ Documentation of staining
- Post-Imaging Processing and Analysis/ Multi-channel fluorescence and DIC image capture
- Post-Imaging Processing and Analysis/ Quantitative morphometry (colorimetric, fluorescent staining) of cells and tissues
- Post-Imaging Processing and Analysis/ Ratio imaging, time-lapse recording
- Post-Imaging Processing and Analysis/ 3-D reconstructions
- Protein analysis (cells and tissues) (Western blot, immunoprecipitation)
- qPCR of specific targets
- Quantitation/ Amino Acids
- Quantitation/ Bile Acids
- Quantitation/ Fatty Acids
- Quantitation/ Glucose & Fructose
- Quantitation of multiple analytes (e.g., cytokines, phosphoproteins) (Luminex (xMAP))
- Quantitation of single analytes (e.g., cytokines) (ELISA)
- Quantitation/ Platelet Activating Factor
- Quantitation/ Prostaglandins
- Quantitation/ Sphingosine-1-P
- Quantitation/ Theobromine
- Quantitation/ Tryptophane
- Regulatory T cells (Tregs) (Multicolor analysis of human and mouse Tregs)
- RNA applications to selective re-sequencing
- Single isolation of cells (Antigen expression (Magnetic bead sorting, MACS))
- Single/multiple sorting of cells (Antigen expression (FACS))
- Sorting of transfected cells (Fluorescent marker/antigen expression (FACS))
- Telomere length (Telomere Flow-FISH)
Ausstattung
AB 3730 (Applied Biosystems)
Affymetrix GCS300 (Affymetrix)
autoMACS (Miltenyi Biotec)
BalTec CPD030 Set Critical Point Dryer
Bioanalyzer 2100 (Agilent)
Bio-Plex 200 (BioRad)
Cell Observer (Zeiss)
Confocal LSM 510 META (Zeiss)
Diatome Diamond Knife 3mm, 45°
FACSAria (BD Biosciences)
FACSCalibur (BD Biosciences)
FAIMS-TSQ Quantum Ultra (Thermo)
Fluorescence Microscope with high speed camera (Zeiss/ Visitron)
for Core Facility services please check www.medunigraz.at/zmf/ua
FPLC
GC- MS
Gelelectrophoresis (BIORAD GE Healthcare)
Genome Sequencer FLX (454/Roche Diagnostics)
HPLC
Imaging System (Aperio Technologies USA/Optoteam Austria)
Leica EM AC20 Ultra Stainer
Leica EM TP
Leica UC6 ultramicrotome
LSR II (BD Biosciences)
LTQ-FT (Thermo)
MagnaPure LC (Roche) & M48 (Qiagen)
MALDI-TOF-TOF (ultrafleXtreme, Bruker)
Mikroscope (Olympus)
Real-time quantitative PCR
TissueFAXS (TissueGnostics)
WAVE/dHPLC (Transgenomics)
ZEISS DSM 950 scanning electron microsope
ZEISS EM 902 transmission electron microscope
4000QTrap (Applied Biosystems)
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