Lehrstuhl für Pharmakologie
Forschungsgebiete, Methoden, Ausstattung
Forschungsgebiete
- Antibiotika induzierte Colitis (Hygiene Fond)
- Cannabinoid chemokine receptor interactions with special focus on viral infection in immune cells (PhD Program
- Drug targeting of endothelium/leukocyte interaction (PhD Program)
- Effect of environmental enrichment on emotional behaviour and the gut-brain axis (BMWF)
- Effect of investigational drugs for irritable bowel syndrome on intestinal circulation (Novartis, Solvay)
- Effect of 5-HT receptor ligands used for irritable bowel syndrome on intestinal circulation (Industrie)
- Evaluation of CRTH2 antagonists (Industrie)
- Evaluation of investigational drugs (opiates, 5-HT receptor ligands) on intestinal motor activity (Industrie)
- Experimental modelling of gastrointestinal inflammation and interoception (BMWF)
- Experimental modelling of irritable bowel syndrome: emphasis on the pain-mood connection and neuroendocrine mechanisms
(in preparation)
- GASP & zellulaerer Transport viraler Chemokinrezeptoren (FWF)
- Gene knockout approach to the neuropharmacology of visceral pain (Nationalbank)
- Klinische Relevanz des CMV kodierten Rezeptors US28 im malignen Melanom (ÖNB)
- Lipid-associated vascular inflammation in atherosclerosis (PhD Program)
- Modulation of eosinophil release from guinea pig bone marrow (Industrie)
- Molecular mechanisms of neurogenic hyperresponsiveness in bronchial asthma (PhD Program)
- Nanoscale functionalities for targeted delivery of biopharmaceutics (EU, Integrated Project)
- Pharmakologie der Leukozyten in Allergie und Entzündung
- Prostaglandin D2 als Mediator der Allergie (FWF)
- Role of kinins in pancreatic pain (in preparation)
- Rolle der Lipidmediatoren Prostaglandin D2, Leukotrien B4 und 5-oxo-ETE in Erkrankungen des oberen Respirationstraktes (ÖNB)
- Sensory and brain responses to gastritis and colitis (FWF)
- Signaling and trafficking properties of oligomerized PGD2 receptors (PhD Program)
- The gastrointestinal nervous system as novel therapeutic target in functional bowel disorders (Zukunftsfonds Steiermark)
- The neuropeptide Y system (neuropeptide Y, peptide YY, Y1, Y2 and Y4 receptors) in its impact on gut pathology, anxiety and stress coping (Zukunftsfonds Steiermark)
- Translational neurogastroenterology (FWF)
Methoden
- BAL- Analyse
- Behavioral analysis of anxiety (open field, elevated plus maze, light-dark box, passive avoidance)
- Behavioral analysis of circadian activity (LabMasterR, homecage activity, drinking, feeding)
- Behavioral analysis of depression (forced swim test, tail suspension test, anhedonia)
- Behavioral analysis of learning and memory (Barnes maze, Morris water maze, object recognition)
- Durchflusszytometrie
- ELISA
- Flow cytometrie d
- Fluorescence microscopy (standard and confocal microscopy)
- Fluorimetrie
- Funktionelle Neuroanatomie
- Gene silencing, transfection and overexpression
- GST-fusion protein/in vitro translation binding assays
- GTPgS assays (High-throughput 96-well with SPA-beads (Scintillation proximity assay))
- HPLC
- Immuncytochemie
- Immunomagnetic cell sorting
- Immunozyto/histo/chemie
- in-situ-Hybridisierung
- in-vitro Darmperistaltik
- in-vitro Rückenmark-Release
- in-vivo/in-vitro Allergisierung
- Isolation of lipoproteins/lipids und acute phase proteins
- Laser-Doppler-Flowmetrie
- Mikrodialyse
- Molecular Biology standard techniques (PCR, site-directed mutagensis, cloning, DNA purification etc.)
- Multiplex ELISA
- Multiplex Fluorescent Bead Immunoassay
- PCR
- Production and affinity purification of polyclonal antibodies
- Protein purification (TAT-fusion proteins, GST-fusion proteins, G protein subunits)
- Radioactive labeling of proteins, lipids and cells
- Radioligand binding assays (High-throughput, 96-well format)
- Real-Time PCR, PCR Array
- RIA, ELISA
- Signal Transduction Pathway Assays (Ca2+ Flux Real Time Assays, Inositol-phosphate turnover, MAP kinases, cAMP, High-throughput 96-well format)
- Transcription-factor assays using Luciferase-reporters (High-throughput, 96-well format)
- Ultrasound Transit-Time Shift Technique
- Verhaltenspharmakologie
- Western blot
- Zellkultur (Primary cultures (Monocytes/macrophages, endothelial cells, mouse embryonic fibroblasts), Cell lines (HEK, COS7, CHO, NIH3T3, Sbcl2, 451Lu, HEp-2)
- Zelluläre Assays (Chemotaxis, Adhesion, Degranulation, Apoptosis, ROS
)
Ausstattung
Aggregometer (Aggrecorder II, KDK)
Analgesiemessung (hot plate, Plantar Test, Algesie-Meter)
Barostat
BD-FACS-Gerät Calibur
Buxco Ganzkörperplethysmographie
CASIE
CO2-Brutschrank
Cryostat
FACS-Gerät BD-FACSCalibur
Flex II Device (Molecular Devices)
FlexiVent Spirometer
Fluoreszenzmikroskope (Olympus & Zeiss) & Image Analyser (CellP & MCID)
FluoStar plate reader
Gel Doc Systems (Biorad)
Isotopenlabors
LabMasterR (TSE)
Mikrotom
Precellys-Tissue- HomegenizerSpezialkäfigsystem MicroVent Typ 84
Real-Time PCR (Eppendorf)
Spezialkäfigsystem MicroVent Typ 84
Tiefkühlschrank HFU
TopCounter (Perkin Elmer)
Ultrazentrifuge
Verhaltenslabors
VideoMot2 Tracking System
Zellkultur-Labors
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