Gottfried Schatz Research Center (for Cell Signaling, Metabolism and Aging)
Research fields, Methods and Equipment
Research Fields
- Adipocytes and obesity
- Ageing and age-related diseases
- Alignment von Stapelbildern, Morphing und Warping Algorithmen
- Bildsegmentierung, Softwareentwicklung zur interaktive Bildverarbeitung
- Bioelektrizität
- Blood-brain barrier
- Cellular lipid homeostasis
- Cellular senescence
- Clinical Chemisry
- Endothelial function and vessel wall function
- Placenta research:
- Analysis of trophoblast invasion
- Trophoblast differentiation and trophoblast fusion
- Turnover and apoptosis of villous trophoblast
- Vascular biology
- Interaction of endothelial cells with mesenchymal stromal cells
- Preeclampsia research:
- Biomarkers to predict preeclampsia in the first trimester pregnancy
- Unravelling the etiology of preeclampsia
- Reproductive immunology:
- Expression and function of HLA-molecules in placental tissue and germ cells with a particular
- emphasis on the non-classical class I molecule HLA-G
- NK-cell functions during pregnancy
- Bone biology and fracture healing
- Neuroscience:
- Neuroanatomy
- Research on the molecular constituents of the nervous system
- Ultrastructure research:
- Analysis of human and non human proteins, cells and tissues
- Functional and clinical anatomy and Biomechanics
- Further important anatomical Essays
- Genetic epidemiology and molecular mechanisms of human aging
- Grundlagenforschung Defibrillieren
- Herz/Kreislaufforschung
- Inflammation
- Injection- and preservation procedure
- Injection-technical investigations at the vessel system
- Insulin/growth factor signalling
- Lipid mediators in the signalling network and in gene expression
- Lipid-and lipoprotein metabolism
- Lipidomics
- Lipotoxicity
- Mathematische Modellierung
- Metabolomics
- Metaboslism
- Modified lipoproteins
- Molecular modelling
- Molecular pharmacology
- Molekulare u. Zelluläre Physiologie
- Mytochondrial dysfunction
- Neurophysiologie
- Nichtlineare Analyse von Bilddaten
- Nuclear import
- Nuclear Magnetic Resonance Spectoscopy
- numerische Simulation
- Optimierung
- Optische Potentialmessung
- Pathomechanisms and prevention of atherosclerosis
- Programmentwicklung
- Radiologic-anatomical investigations of cavaties and canal systems of the skull
- Radiologic-anatomical investigations of the active and passive apparatus of locomotion
- Radiologic-anatomical investigations of the vessel and nervous systems
- Rational drug design
- Redox signalling
- Regulation of cellular Ion equilibrium
- RNA-binding proteins
- SAXS
- Signalling
- Signaltransduktion innerhalb u. zwischen Zellen
- Sonographical investigations of the active and passive apparatus of locomotion
- Sonographical investigations of the vessel and nervous systems
- Statistical Genetics
- Systemidentifikation
- The role of Ca++ in cell function
- type 2 diabetes mellitus
- Wnt signaling
- Zelluläre Elektrophysiologie des menschlichen Herzens
Methods
- Animal experiments
- Biophysical interaction studies
- Cell culture
- Chromatography
- Computersimulation
- Computersimulation der zellulären Erregungsvorgänge
- Corrosion casting
- Cutting techniques
- Differentialgleichungssysteme
- Dissection (skillful incision and exploration of body cavities and their organs)
- DNA cloning
- Electrophoresis
- Elektrophysiologie
- Encyme activities
- FIR / FRET / in-vivo Mikroskopie an Fluoreszenzproteinen
- Gene expression
- Genexpression / Site directed mutagenesis / rekombinante Proteine / Genkonstrukte /
- Gentechnik
- Herzteilpräparate
- High throughput DNA/RNA Analysis
- Imaging methods (radiology, computer tomography, magnetic resonance tomography, sonography, szintigraphie, etc.
- In vitro and in vivo gene transfer
- In-vitro u. in-vivo Phosphorylierung / protein;protein Interaktion mittels pull down assay / Immunprezipitation / in-vitro Translation
- isolierte Zellen
- Konfokale LS Mikroskopie
- Maceration of bones
- Mathematische Methoden
- Measurement
- Metabolomics
- Micro arrays
- Microscopy: Confocal laser scanning microscopy, Deconvolution microscopy
- Molecular modelling
- Next generation sequencing
- NMR spektroscopy
- Northern Blot analysis
- numerische Lösung mittels Algorithmen
- Optische Spannungsmessungen
- Palpation
- Patch clamp
- PCR, RT-PCR
- Pharmakologie
- Plastination
- Preparation (skillful exposition und resection of the parts)
- Preservation (skillful conservation and maintaining of biological material by chemical procedures)
- Production of antibodies
- Programmiersprachen, insbesondere Matlab und C
- Programming
- Protein expression and purification
- Protein labelling
- Proteinbiochemie
- Proteomics
- real time PCR
- siRNA
- Small-angle scattering
- Softwareentwicklung
- Southern Blot
- Transfection of mammalian cells
- Voltage clamp
- Western Blot
- wire myography
- Zellisolation
- Zellkultur
- zwei Elektroden Voltage Clamp /Patch Clamp
- Histological methods:
- Light microscopy (fixation, paraffin embedding, cryo-sectioning, different staining techniques)
- Immunohistochemistry (fluorochrome and enzymatic detection, immunogold-labeling)
- Scanning electron microscopy
- Transmission electron microscopy (thin sections techniques, replica technology e.g. freeze etch and freeze fracture, immunogold-labelling using pre- and post-embedding methods)
- 3D-electron microscopy using both serial sections and electron tomography
- Confocal laser scanning microscopy
- Laser microdissection
- Single cell analysis
Methods in Molecular and Cell Biology:- DNA and RNA extraction from cells and tissues
- Southern and Northern blotting (radioactive, non-radioactive)
- In situ Hybridization (non-radioactive)
- PCR, RT-PCR and qPCR
- FACS analysis
Biochemical methods:- 1- and 2-dimensional Polyacrylamide-Gelelectrophoresis
- Western blotting
- ELISA
Cell and Tissue Culture:- Culture of several non-transfected and transfected cell lines (e.g. choriocarcinoma cells)
- Isolation and culture of feta cell from human placenta (e.g. different trophoblast and endothelial cell populations, mesenchymal stromal cells)
- Isolation and culture of cells from human blood vessels (endothelial cells, fibroblasts)
- Bioreactor model for the development of vascular protheses
- 3-dimensional confrontation culture
- Culture of cells and tissues under various oxygen concentrations (0.1% to 90%)
- Live cell imaging with phase contrast and fluorescence markers
- Production of monoclonal antibodies
Equipment
blood pressure gauge - mouse,rat
confocal array scanning microscope
Gaschromatograph
GC-MS
Gel documentation system
HPLC
In-house SAXS machine (Anton Paar SAXSpace) equipped with a temperature-controlled autosampler (up to 2 x 96 samples), various sample holders, and detectors
Patch Clamp Technic (also applicable for all microscopes)
Pipetting robot (AndrewAlliance 1000P)
Pipetting roboter (Janus, PE)
Protein expression and purification platform, including FPLC (GE Healthcare Acta Pure)
Real Time PCR (ABI 7900)
ultra centrifuges
wire myography system
600 MHz NMR spetrometer (Bruker Biospin) equipped with BBI/TXI probeheads and a temperature-controlled autosampler (up to 500 samples)
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